Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-13 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.392, 88.200, 70.936 |
| Unit cell angles | 90.00, 103.41, 90.00 |
Refinement procedure
| Resolution | 37.160 - 2.550 |
| R-factor | 0.1853 |
| Rwork | 0.183 |
| R-free | 0.22470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gg2 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.810 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MR-Rosetta |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.590 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.160 | 0.930 |
| Number of reflections | 27596 | |
| <I/σ(I)> | 10.6 | 1.74 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 297 | 0.1 M magnesium formate, 15% PEG 3350, pH 8.0, Temperature 297K |
