4XRP

Structure of the Pnkp1/Rnl/Hen1 RNA repair complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-06
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9794
Spacegroup name	P 1 21 1
Unit cell lengths	111.160, 179.201, 114.333
Unit cell angles	90.00, 103.70, 90.00

Refinement procedure

Resolution	47.206 - 3.300
R-factor	0.1767
Rwork	0.175
R-free	0.23810
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: dev_1624))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.300
Number of reflections	65439
<I/σ(I)>	17.1
Completeness [%]	98.4
Redundancy	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	278	PEG 6000