4XRH
Human DNA polymerase lambda- MgdTTP binary and complex with 6 paired DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2013-10-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 84.083, 84.083, 397.760 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.375 - 3.000 |
| R-factor | 0.2213 |
| Rwork | 0.218 |
| R-free | 0.25140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xsl |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.665 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.440 | 3.000 |
| Rmerge | 0.153 | 0.045 | 0.701 |
| Total number of observations | 98387 | ||
| Number of reflections | 17723 | ||
| <I/σ(I)> | 5.8 | ||
| Completeness [%] | 99.2 | 97.2 | 99.8 |
| Redundancy | 5.6 | 4.9 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1M Lithium Sulfate monohydrate, 0.1M HEPES-Sodium, 0.1M Potassium Sodium Tartrate |
