4XRE
Crystal structure of Gnk2 complexed with mannose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2011-11-26 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 3 |
Unit cell lengths | 143.400, 143.400, 143.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.886 - 2.597 |
R-factor | 0.2511 |
Rwork | 0.249 |
R-free | 0.28850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3a2e |
RMSD bond length | 0.007 |
RMSD bond angle | 1.132 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.640 |
High resolution limit [Å] | 2.597 | 2.600 |
Number of reflections | 30598 | |
<I/σ(I)> | 74.7 | 9.9 |
Completeness [%] | 99.9 | 100 |
Redundancy | 21.5 | 20.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 28% PEG MME 2000, 0.1M TRIS-HCL, 0.2M AMMONIUM SULFATE |