4XQU

Crystal structure of hemagglutinin from Jiangxi-Donghu (2013) H10N8 influenza virus in complex with 3'-SLN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-17
Detector	PSI PILATUS 6M
Wavelength(s)	1.0716
Spacegroup name	P 1 21 1
Unit cell lengths	64.874, 245.853, 70.995
Unit cell angles	90.00, 112.46, 90.00

Refinement procedure

Resolution	48.386 - 3.250
R-factor	0.2193
Rwork	0.217
R-free	0.26120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xq5
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.310
High resolution limit [Å]	3.250	8.810	3.250
Rmerge	0.146	0.056	0.569
Rmeas	0.180	0.069	0.727
Rpim	0.102	0.039	0.448
Total number of observations	86794
Number of reflections	31215
<I/σ(I)>	5
Completeness [%]	95.9	95.3	91.7
Redundancy	2.8	2.9	2.1
CC(1/2)		0.995	0.650

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277.15	0.2 M NaSCN, 20% (w/v) PEG 3350