4XQN
Crystal structure of AgrA LytTR domain in complex with promoters
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-08 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.95373 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.170, 61.320, 64.100 |
| Unit cell angles | 91.09, 97.48, 94.02 |
Refinement procedure
| Resolution | 44.690 - 2.300 |
| R-factor | 0.21303 |
| Rwork | 0.211 |
| R-free | 0.24750 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.461 |
| Data reduction software | SCALA |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.690 | 2.380 |
| High resolution limit [Å] | 2.260 | 2.300 |
| Rmerge | 0.560 | |
| Number of reflections | 29349 | |
| <I/σ(I)> | 12.6 | 2.7 |
| Completeness [%] | 97.1 | 96.3 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.1M Sodium citrate tribasic dihydrate pH 5.6, 20% 2-propanol, 20% PEG 400 |
