4XQ8
Human DNA polymerase lambda- MgdATP binary complex and complex with 6 paired DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2013-10-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 83.743, 83.743, 398.746 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.411 - 2.798 |
| R-factor | 0.187 |
| Rwork | 0.182 |
| R-free | 0.23990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4W5D |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.183 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.900 |
| High resolution limit [Å] | 2.798 | 6.020 | 2.800 |
| Rmerge | 0.084 | 0.026 | 0.491 |
| Rmeas | 0.089 | 0.028 | 0.526 |
| Rpim | 0.031 | 0.010 | 0.187 |
| Total number of observations | 178549 | ||
| Number of reflections | 21679 | ||
| <I/σ(I)> | 9.5 | ||
| Completeness [%] | 100.0 | 99.7 | 100 |
| Redundancy | 8.2 | 7.4 | 7.8 |
| CC(1/2) | 1.000 | 0.902 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 0.1 M Lithium Sulfate monohydrate, 0.1 M HEPES-Sodium, 0.1 M Potassium Sodium Tartrate |
