4XO5
Crystal Structure of E. coli Aminopeptidase N in complex with L-Glutamate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-01-06 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 120.482, 120.482, 170.424 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.590 - 1.980 |
| R-factor | 0.12961 |
| Rwork | 0.128 |
| R-free | 0.15844 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hpo |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.963 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.050 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Number of reflections | 99371 | |
| <I/σ(I)> | 16.95 | 3.64 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.2 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | Sodium malonate |
