4XN5

Crystal Structure of Met260Ala mutant of E. coli Aminopeptidase N in complex with L-Phenylalanine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-03-01
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 31 2 1
Unit cell lengths	121.033, 121.033, 170.808
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	17.970 - 2.660
R-factor	0.1428
Rwork	0.140
R-free	0.20224
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hpo
RMSD bond length	0.015
RMSD bond angle	1.746
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.750
High resolution limit [Å]	2.660	2.660
Number of reflections	45125
<I/σ(I)>	7.39	2.43
Completeness [%]	98.7	96.7
Redundancy	5.6	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	Sodium malonate