4XMX
Crystal Structure of Met260Ala mutant of E. coli Aminopeptidase N in complex with Bestatin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-02-21 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 121.037, 121.037, 170.658 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.810 - 2.300 |
R-factor | 0.14739 |
Rwork | 0.145 |
R-free | 0.19131 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hpo |
RMSD bond length | 0.019 |
RMSD bond angle | 1.871 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.810 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 64564 | |
<I/σ(I)> | 9.94 | 3.02 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.5 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | Sodium malonate |