4XMM
Structure of the yeast coat nucleoporin complex, space group C2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-08-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9794 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 210.645, 186.297, 199.570 |
Unit cell angles | 90.00, 100.85, 90.00 |
Refinement procedure
Resolution | 67.516 - 7.384 |
R-factor | 0.3322 |
Rwork | 0.330 |
R-free | 0.35270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3iko 3f3f 3f7f and 3PGF |
RMSD bond length | 0.006 |
RMSD bond angle | 1.217 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: dev_1809)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.000 | 7.800 |
High resolution limit [Å] | 7.384 | 7.384 |
Rmerge | 0.097 | 0.212 |
Number of reflections | 10375 | |
<I/σ(I)> | 15.3 | 1.4 |
Completeness [%] | 99.0 | 94.9 |
Redundancy | 13.5 | 13.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | PEG 20000, ethanol, MES |