4XMM

Structure of the yeast coat nucleoporin complex, space group C2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9794
Spacegroup name	C 1 2 1
Unit cell lengths	210.645, 186.297, 199.570
Unit cell angles	90.00, 100.85, 90.00

Refinement procedure

Resolution	67.516 - 7.384
R-factor	0.3322
Rwork	0.330
R-free	0.35270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3iko 3f3f 3f7f and 3PGF
RMSD bond length	0.006
RMSD bond angle	1.217
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1809))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.000	7.800
High resolution limit [Å]	7.384	7.384
Rmerge	0.097	0.212
Number of reflections	10375
<I/σ(I)>	15.3	1.4
Completeness [%]	99.0	94.9
Redundancy	13.5	13.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	PEG 20000, ethanol, MES