4XKJ

a Novel D-lactate Dehydrogenase from Sporolactobacillus sp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-04-14
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	61.263, 109.505, 113.003
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.534 - 3.148
R-factor	0.2133
Rwork	0.204
R-free	0.29220
Starting model (for MR)	2yq4
RMSD bond length	0.009
RMSD bond angle	1.439
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.200
High resolution limit [Å]	3.148	3.150
Rmerge		0.290
Number of reflections	13247
<I/σ(I)>	9.4
Completeness [%]	96.4
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	Ammonium acetate,Sodium citrate tribasic dihydrate,Polyethylene glycol 4000