4XJN
Structure of the parainfluenza virus 5 nucleocapsid-RNA complex: an insight into paramyxovirus polymerase activity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.95041 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 205.636, 309.436, 233.241 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.007 - 3.110 |
| R-factor | 0.2288 |
| Rwork | 0.227 |
| R-free | 0.26250 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 171.300 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.178 | |
| Number of reflections | 132485 | |
| <I/σ(I)> | 11.8 | 1.475 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 7 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | Crystlliztion Condition: 30% PEG 400, 0.1M MES sodium salt pH 6.5, 0.1 M MgCl2. Crystals grew from 4 days to one week, and varied in size from 50um to 400um |
