4XI5
gHgL of varicella-zoster virus in complex with human neutralizing antibodies
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.00000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 102.002, 280.552, 175.302 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.322 - 3.900 |
R-factor | 0.2467 |
Rwork | 0.245 |
R-free | 0.27640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xhj |
RMSD bond length | 0.003 |
RMSD bond angle | 0.944 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 200.000 | 3.970 |
High resolution limit [Å] | 3.885 | 3.885 |
Rmerge | 0.067 | 0.753 |
Number of reflections | 23170 | |
<I/σ(I)> | 24.4 | 1.7 |
Completeness [%] | 99.1 | |
Redundancy | 6.3 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1M MES pH 6.0 and 4% PEG 4000 |