4XHJ

gHgL of Varicella-zoster virus in complex with human neutralizing antibodies.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00000
Spacegroup name	P 43
Unit cell lengths	147.001, 147.001, 198.328
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.104 - 3.156
R-factor	0.2383
Rwork	0.236
R-free	0.27830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3m1c
RMSD bond length	0.004
RMSD bond angle	1.181
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1839))

Data quality characteristics

	Overall
Low resolution limit [Å]	200.000
High resolution limit [Å]	3.150
Rmerge	0.076
Number of reflections	71938
<I/σ(I)>	22.1
Completeness [%]	99.9
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M MES pH 6.5, 9% PEG 3350 and 0.2M MgCl2