4XH2
Crystal structure of human paxillin LD4 motif in complex with Fab fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-14 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 117.580, 138.120, 223.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 117.500 - 2.000 |
| R-factor | 0.226 |
| Rwork | 0.224 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pgf |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.169 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 117.500 | 2.200 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.155 | 0.997 |
| Number of reflections | 228060 | |
| <I/σ(I)> | 7.6 | 1.76 |
| Completeness [%] | 93.2 | 84.2 |
| Redundancy | 4.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 18% PEG 8000, 0.1 M MES, 0.5 % n-Dodecyl-N,N-Dimethylamine-N-Oxide (LDAO) |
