4XGO

Crystal structure of leucine-rich repeat domain of APL1B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-03-11
Detector	PSI PILATUS 6M
Wavelength(s)	1.1
Spacegroup name	P 21 21 21
Unit cell lengths	64.423, 74.956, 214.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	107.180 - 1.740
R-factor	0.18822
Rwork	0.187
R-free	0.21964
RMSD bond length	0.008
RMSD bond angle	1.314
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	107.180	1.790
High resolution limit [Å]	1.740	1.740
Rmerge		0.530
Number of reflections	91036
<I/σ(I)>	36.7	2.6
Completeness [%]	89.9	51.05
Redundancy	6.1	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	293	0.1 M SODIUM ACETATE, 0.1 M LITHIUM SULFATE, 1.0 M AMMONIUM PHOSPHATE