4XG1
Psychromonas ingrahamii diaminopimelate decarboxylase with LLP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.960, 103.130, 93.590 |
| Unit cell angles | 90.00, 94.77, 90.00 |
Refinement procedure
| Resolution | 19.850 - 2.500 |
| R-factor | 0.2119 |
| Rwork | 0.211 |
| R-free | 0.24589 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 3N2B |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.506 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX (Auto-Rickshaw web server) |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.170 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.072 | 0.146 |
| Number of reflections | 58922 | |
| <I/σ(I)> | 20.3 | 11.6 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 7.3 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 281 | 28%(W/V) PEG 3350, 100mM BIS-TRIS PROPANE (PH 6.0), 200mM POTASSIUM THIOCYANATE, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 281K |
