4XF2
Tetragonal structure of Arp2/3 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | P 41 |
Unit cell lengths | 149.930, 149.930, 265.911 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.536 - 5.000 |
R-factor | 0.2762 |
Rwork | 0.275 |
R-free | 0.30310 |
Structure solution method | MIR |
Starting model (for MR) | 1U2V with the calcium and ADP ligands removed |
RMSD bond length | 0.011 |
RMSD bond angle | 2.365 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 |
High resolution limit [Å] | 5.000 | 5.000 |
Number of reflections | 24560 | |
<I/σ(I)> | 20.3 | 2.3 |
Completeness [%] | 96.8 | 84.6 |
Redundancy | 6.4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 100 mM Tris 7.0, 15% ethanol |