4XF0
cysteine dioxygenase variant - C93A at pH 8.0 with cysteine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.977 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 57.600, 57.600, 122.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.907 - 1.399 |
R-factor | 0.1583 |
Rwork | 0.155 |
R-free | 0.18600 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.277 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.000 | 1.420 |
High resolution limit [Å] | 1.399 | 1.399 |
Number of reflections | 37752 | |
<I/σ(I)> | 22.5 | 1.8 |
Completeness [%] | 91.8 | 54.1 |
Redundancy | 28.1 | 26.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | 0.1M tri-sodium citrate pH=5.6, 24% PEG 4000, 0.15M ammonium acetate. 1:1 drop ratio with microseeding.; Soak solution made with addition of 100mM Cys at pH 8.0 |