4XE5
Crystal structure of the Na,K-ATPase from bovine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-05-26 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 64.750, 301.070, 242.290 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.740 - 3.901 |
R-factor | 0.3231 |
Rwork | 0.322 |
R-free | 0.33630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hyt |
RMSD bond length | 0.006 |
RMSD bond angle | 0.906 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1888) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | |
High resolution limit [Å] | 3.700 | 3.700 |
Rmerge | 0.157 | |
Number of reflections | 15157 | |
<I/σ(I)> | 9.6 | |
Completeness [%] | 59.2 | |
Redundancy | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | PEG 3,350, manganese chloride, 3-(N-morpholino)propansulfonic acid (pH adjusted with N-Methyl-D-glucamin), 2-Mercaptoethanol, Octaethylene glycol monododecyl ether |