4XDP
Crystal structure of human KDM4C catalytic domain bound to tris
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-15 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 93.870, 89.750, 98.280 |
Unit cell angles | 90.00, 96.31, 90.00 |
Refinement procedure
Resolution | 31.420 - 2.070 |
R-factor | 0.2084 |
Rwork | 0.207 |
R-free | 0.24200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.367 |
Data reduction software | XDS |
Data scaling software | Aimless (0.1.29) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 31.420 | 31.420 | 2.120 |
High resolution limit [Å] | 2.070 | 9.260 | 2.070 |
Rmerge | 0.124 | 0.027 | 0.669 |
Rpim | 0.068 | 0.016 | 0.366 |
Total number of observations | 209621 | 2160 | 15403 |
Number of reflections | 48744 | ||
<I/σ(I)> | 11 | 35.8 | 2.3 |
Completeness [%] | 98.6 | 97.3 | 97.8 |
Redundancy | 4.3 | 3.9 | 4.3 |
CC(1/2) | 0.995 | 0.999 | 0.733 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291.15 | 0.8 M Lithium chloride, 0.1 M TRIS pH 8.5, 28 % w/v PEG 4000 |