4XCY

Crystal structure of human 4E10 Fab in complex with phosphatidylglycerol (06:0 PG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	110
Detector technology	PIXEL
Collection date	2013-06-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00000
Spacegroup name	P 61 2 2
Unit cell lengths	150.220, 150.220, 472.160
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.907 - 3.960
R-factor	0.246
Rwork	0.244
R-free	0.29100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fx7
RMSD bond length	0.004
RMSD bond angle	0.988
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (2.5.2)
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.907	4.060
High resolution limit [Å]	3.960	3.960
Rmerge		0.711
Number of reflections	27268
<I/σ(I)>	5.2	2.1
Completeness [%]	95.5	97.4
Redundancy	4.43	4.16

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	293	DROP CONTAINS 10 MG/ML 4E10 FAB, 10 MM PHOSPHATIDYLGLYCEROL (06:0 PG) MIXED 1:1 V/V WITH RESERVOIR SOLUTION OF 0.2 M SODIUM IODIDE, 20% PEG 3350, PH 6.0, VAPOR DIFFUSION, TEMPERATURE 293K