4XCN
Crystal structure of human 4E10 Fab in complex with phosphatidic acid (06:0 PA); 2.9 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2013-05-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 149.890, 149.890, 469.760 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.063 - 2.900 |
| R-factor | 0.1952 |
| Rwork | 0.193 |
| R-free | 0.24640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fx7 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.836 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.5.2) |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.063 | 2.970 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.864 | |
| Number of reflections | 68834 | |
| <I/σ(I)> | 12 | 3.2 |
| Completeness [%] | 98.1 | 97.7 |
| Redundancy | 13.1 | 13.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | Drop contains 10 mg/ml 4E10 Fab, 10 mM 06:0 PA mixed 1:1 v/v with a reservoir solution of 0.2 M potassium formate, 20% PEG 3350 |
