4XCC
Crystal structure of human 4E10 Fab crystalized in the presence of Phosphatidylcholine (06:0 PC); I422 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2013-07-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 108.071, 108.071, 186.247 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.781 - 2.810 |
| R-factor | 0.2239 |
| Rwork | 0.221 |
| R-free | 0.27220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fx7 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.637 |
| Data reduction software | HKL-2000 (HKL-2000 version 705b) |
| Data scaling software | HKL-2000 (HKL-2000 version 705b) |
| Phasing software | PHASER (2.5.2) |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.781 | 2.860 |
| High resolution limit [Å] | 2.810 | 2.810 |
| Rmerge | 0.593 | |
| Number of reflections | 12759 | |
| <I/σ(I)> | 16 | 5.3 |
| Completeness [%] | 92.1 | 95.1 |
| Redundancy | 8.6 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | Sitting drops contain 4E10 Fab at 10 mg/ml, 10 mM Phosphatidylcholine (06:0 PC) sample mixed (1:1 v/v) with a reservoir solution of 0.2 M di-sodium tartrate, 20% PEG 3350 |
