4XC9
Crystal Structure of apo HygX from Streptomyces hygroscopicus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-23 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 112.113, 274.427, 52.825 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.295 - 2.400 |
R-factor | 0.1868 |
Rwork | 0.183 |
R-free | 0.24580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xca |
RMSD bond length | 0.008 |
RMSD bond angle | 1.120 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 5.170 | 2.400 |
Rmerge | 0.080 | 0.046 | 0.443 |
Total number of observations | 228727 | ||
Number of reflections | 61662 | ||
<I/σ(I)> | 9.7 | ||
Completeness [%] | 92.9 | 98.7 | 91.1 |
Redundancy | 3.7 | 4.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 100 mM Bis-Tris, 100 mM MgCl2, 12% PEG8000 |