4XC3
Crystal structure of human 4E10 Fab in complex with its peptide epitope on HIV-1 gp41; crystals cryoprotected with rac-glycerol 1-phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2011-04-12 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.00000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 157.541, 44.704, 85.942 |
Unit cell angles | 90.00, 113.22, 90.00 |
Refinement procedure
Resolution | 32.796 - 1.630 |
R-factor | 0.139 |
Rwork | 0.137 |
R-free | 0.17990 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.062 |
Data reduction software | HKL-2000 (0.98.701) |
Data scaling software | HKL-2000 (0.98.701) |
Phasing software | PHASER (2.1.4) |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.796 | 1.660 |
High resolution limit [Å] | 1.630 | 1.630 |
Rmerge | 0.451 | |
Number of reflections | 67194 | |
<I/σ(I)> | 20.47 | 2.1 |
Completeness [%] | 97.3 | 74.2 |
Redundancy | 4 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.5 | 293 | The complex grew from 1:1 protein:reservoir solution sitting drops equilibrated against 20% PEG 8000, 0.2 M sodium acetate, pH 5.5, 0.2 M sodium thiocyanate |