4XBM

X-ray crystal structure of Notch ligand Delta-like 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	108.150, 118.870, 134.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.300 - 3.200
R-factor	0.2601
Rwork	0.258
R-free	0.28350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cbz
RMSD bond length	0.004
RMSD bond angle	0.928
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.400	3.314
High resolution limit [Å]	3.200	3.200
Rmerge	0.432
Number of reflections	29271
<I/σ(I)>	6.27
Completeness [%]	99.9
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.4M potassium thiocyanate 16% PEG3350 0.1M Bis-tris propane pH 7.5 5mM CaCl2