4XB1

Hyperthermophilic archaeal homoserine dehydrogenase in complex with NADPH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-25
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0
Spacegroup name	P 41
Unit cell lengths	112.560, 112.560, 95.885
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.300
R-factor	0.1705
Rwork	0.169
R-free	0.20460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3do5
RMSD bond length	0.020
RMSD bond angle	2.133
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	DM

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	53097
<I/σ(I)>	21.4	9.1
Completeness [%]	99.8	99.9
Redundancy	7.6	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	293	2-methyl-2,4-pentandiol, potassium phosphate