4XAS
mGluR2 ECD ligand complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-13 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.97931 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 78.044, 135.858, 92.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.700 - 2.350 |
R-factor | 0.2144 |
Rwork | 0.212 |
R-free | 0.28010 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.120 |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER-TNT (BUSTER 2.11.5) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 19.700 |
High resolution limit [Å] | 2.350 |
Number of reflections | 40851 |
<I/σ(I)> | 8 |
Completeness [%] | 98.5 |
Redundancy | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 295 | 100mM Sodium Acetate pH 4.6 + 23% PEG 10K |