4XAS

mGluR2 ECD ligand complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-13
Detector	RAYONIX MX-225
Wavelength(s)	0.97931
Spacegroup name	P 21 21 2
Unit cell lengths	78.044, 135.858, 92.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.700 - 2.350
R-factor	0.2144
Rwork	0.212
R-free	0.28010
RMSD bond length	0.010
RMSD bond angle	1.120
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER-TNT (BUSTER 2.11.5)

Data quality characteristics

	Overall
Low resolution limit [Å]	19.700
High resolution limit [Å]	2.350
Number of reflections	40851
<I/σ(I)>	8
Completeness [%]	98.5
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		295	100mM Sodium Acetate pH 4.6 + 23% PEG 10K