4XAQ
mGluR2 ECD and mGluR3 ECD with ligands
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-17 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.97929 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 81.644, 159.652, 93.880 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.520 - 2.210 |
R-factor | 0.1877 |
Rwork | 0.186 |
R-free | 0.22470 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.030 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER-TNT (BUSTER 2.11.5) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.520 |
High resolution limit [Å] | 2.210 |
Number of reflections | 61903 |
<I/σ(I)> | 8.6 |
Completeness [%] | 99.0 |
Redundancy | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 295 | 100mM MES pH 6 + 25% PEG 4K + 200mM Ammonium Sulfate |