4X9C

1.4A crystal structure of Hfq from Methanococcus jannaschii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.86
Spacegroup name	P 21 21 21
Unit cell lengths	57.667, 66.857, 109.212
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.667 - 1.400
R-factor	0.1673
Rwork	0.166
R-free	0.18770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qtx
RMSD bond length	0.006
RMSD bond angle	1.070
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.051	0.661
Number of reflections	83538
<I/σ(I)>	21.8	2.85
Completeness [%]	99.7	97.3
Redundancy	6.5	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	296	A2 JBCScreen Nuc-Pro 1 (50% PEG200, 0,1M Tris-HCl, pH 8,0)