4X9C
1.4A crystal structure of Hfq from Methanococcus jannaschii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-11-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.86 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.667, 66.857, 109.212 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.667 - 1.400 |
R-factor | 0.1673 |
Rwork | 0.166 |
R-free | 0.18770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qtx |
RMSD bond length | 0.006 |
RMSD bond angle | 1.070 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.450 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.051 | 0.661 |
Number of reflections | 83538 | |
<I/σ(I)> | 21.8 | 2.85 |
Completeness [%] | 99.7 | 97.3 |
Redundancy | 6.5 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 296 | A2 JBCScreen Nuc-Pro 1 (50% PEG200, 0,1M Tris-HCl, pH 8,0) |