4X99

Immunoglobulin Fc heterodimers variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 61 2 2
Unit cell lengths	152.766, 152.766, 109.981
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.628 - 2.498
R-factor	0.2512
Rwork	0.248
R-free	0.28330
Starting model (for MR)	3ave
RMSD bond length	0.013
RMSD bond angle	1.564
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.498	2.500
Rmerge	0.010
Number of reflections	26777
<I/σ(I)>	58.6	5.5
Completeness [%]	99.8	99.5
Redundancy	15.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	3M NaCl, 0.1M sodium acetate, pH 4.5