4X98

Immunoglobulin Fc heterodimer variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 61 2 2
Unit cell lengths	152.744, 152.744, 108.984
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.840 - 2.499
R-factor	0.2311
Rwork	0.228
R-free	0.27440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ave
RMSD bond length	0.011
RMSD bond angle	1.429
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.499	2.500
Rmerge	0.095
Number of reflections	26500
<I/σ(I)>	59.9
Completeness [%]	99.9	99.42
Redundancy	16.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	3M NaCl, 0.1M sodium acetate, pH 4.5