4X90
Crystal structure of Lysosomal Phospholipase A2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 1 |
| Unit cell lengths | 62.806, 91.151, 100.266 |
| Unit cell angles | 78.13, 88.46, 88.50 |
Refinement procedure
| Resolution | 30.000 - 1.840 |
| R-factor | 0.155 |
| Rwork | 0.154 |
| R-free | 0.17330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.441 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.860 |
| High resolution limit [Å] | 1.830 | 4.960 | 1.830 |
| Rmerge | 0.095 | 0.058 | 0.631 |
| Total number of observations | 726111 | ||
| Number of reflections | 183439 | ||
| <I/σ(I)> | 6.7 | ||
| Completeness [%] | 97.8 | 99.5 | 96.4 |
| Redundancy | 4 | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 100 mM HEPES pH 7.5, 3.5% PEG 8000, 28% MPD, 300 mM (NH4)2HPO4 |
