4X7F
Crystal structure of norovirus GII.10 P domain in complex with Nano-25
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-09-09 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.984 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.280, 112.980, 142.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.587 - 1.700 |
R-factor | 0.1612 |
Rwork | 0.160 |
R-free | 0.18680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | As search models for molecular replacement PDB code 3ONU was used for GII.10 P domain (molecule 1) and PDB code 3P0G for Nano-25 (molecule 2) |
RMSD bond length | 0.010 |
RMSD bond angle | 1.191 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.590 | 1.800 | |
High resolution limit [Å] | 1.700 | 7.600 | 1.700 |
Rmerge | 0.056 | 0.031 | 0.355 |
Rmeas | 0.067 | 0.038 | 0.487 |
Total number of observations | 412753 | ||
Number of reflections | 88133 | 1891 | 11178 |
<I/σ(I)> | 14.33 | 20.82 | 2.58 |
Completeness [%] | 99.1 | 98.4 | 96.8 |
Redundancy | 4.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | PEG3350, ammonium dihydrogen phosphate |