4X62

Crystal Structure of 30S ribosomal subunit from Thermus thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 41 21 2
Unit cell lengths	400.834, 400.834, 175.086
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.884 - 3.449
R-factor	0.1826
Rwork	0.181
R-free	0.21430
RMSD bond length	0.004
RMSD bond angle	0.753
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: dev_1938))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	3.510
High resolution limit [Å]	3.449	9.310	3.449
Rmerge	0.160	0.072	0.937
Rmeas	0.168	0.076	0.984
Rpim	0.051	0.023	0.297
Total number of observations	1935258
Number of reflections	184085
<I/σ(I)>	5.4
Completeness [%]	99.2	96.3	100
Redundancy	10.5	10.7	10.7
CC(1/2)		0.997	0.854

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	MPD