4X5U
X-ray crystal structure of CagL at pH 4.2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979450 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 63.018, 63.018, 243.763 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 54.580 - 2.300 |
R-factor | 0.25734 |
Rwork | 0.254 |
R-free | 0.31283 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zci |
RMSD bond length | 0.008 |
RMSD bond angle | 1.094 |
Data scaling software | XDS |
Refinement software | REFMAC (5.8.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.580 | 2.390 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.169 | 5.247 |
Number of reflections | 13696 | |
<I/σ(I)> | 19.3 | 1.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 37.2 | 39.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | 30% v/v ethanol, 200 mM sodium phosphate/citric acid, pH 4.2, 5% w/v PEG 1000, 1% v/v glycerol, Microseeding |