4X5H

ecDHFR complexed with folate and NADP+ at 500 MPa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	298
Detector technology	CCD
Collection date	2013-06-18
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.75
Spacegroup name	C 1 2 1
Unit cell lengths	74.182, 58.636, 38.284
Unit cell angles	90.00, 106.78, 90.00

Refinement procedure

Resolution	36.680 - 1.900
R-factor	0.16317
Rwork	0.160
R-free	0.22222
RMSD bond length	0.019
RMSD bond angle	2.007
Data scaling software	HKL-2000
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.900
Number of reflections	12674
<I/σ(I)>	27
Completeness [%]	98.5
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	PEG 6000, imidazole, calcium chloride