4X4Z
Retrofitting antibodies with stabilizing mutations. Herceptin VL mutant F53D.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.954 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 98.580, 103.750, 110.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.770 - 1.800 |
| R-factor | 0.1788 |
| Rwork | 0.177 |
| R-free | 0.21264 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.984 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.770 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.075 | 0.428 |
| Number of reflections | 26083 | |
| <I/σ(I)> | 7.9 | 2.4 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 3.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1.1 M Sodium Malonate, 100 mM HEPES (pH 7.0), 1% (v/v) Jeffamine ED-2003 |
