4X4W

Crystal structure of the full-length human mitochondrial CCA-adding enzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-12-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.1
Spacegroup name	P 1 21 1
Unit cell lengths	40.970, 142.360, 100.590
Unit cell angles	90.00, 94.64, 90.00

Refinement procedure

Resolution	29.076 - 1.900
R-factor	0.156
Rwork	0.154
R-free	0.19580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.572
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]			1.950
High resolution limit [Å]	1.900	8.500	1.900
Rmerge	0.049	0.016	0.536
Rmeas	0.059	0.019	0.650
Total number of observations	271414
Number of reflections	89161	994	6668
<I/σ(I)>	15.69	51.55	2.33
Completeness [%]	98.9	95.3	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	290	2.2M Ammonium Sulfate, 0.05M Na Citrate pH 5.5