4X3K
Crystal structure of chromobox homolog 7 (CBX7) chromodomain with H3K27me3 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-04 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.460, 33.230, 46.160 |
| Unit cell angles | 90.00, 95.80, 90.00 |
Refinement procedure
| Resolution | 30.710 - 1.450 |
| R-factor | 0.19089 |
| Rwork | 0.189 |
| R-free | 0.23213 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.346 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.710 | 1.520 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 24687 | |
| <I/σ(I)> | 18.3 | 5.8 |
| Completeness [%] | 99.4 | 99 |
| Redundancy | 7.2 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | 25% PEG MME550, 0.1 M MES pH6.5, 0.01 M zinc sulfate |
