4X3E

Crystal structure of EED in complex with a trimethylated Jarid2 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Collection date	2011-08-05
Detector	ADSC QUANTUM 4r
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	49.419, 58.144, 127.042
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.072 - 2.300
R-factor	0.1736
Rwork	0.171
R-free	0.22390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3iiw
RMSD bond length	0.007
RMSD bond angle	1.074
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.090
High resolution limit [Å]	2.000	2.000
Number of reflections	16463
<I/σ(I)>	16.2	2.23
Completeness [%]	97.2	38.2
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	Sodium Formate