4X1Q
The crystal structure of mupain-1 in complex with murinised human uPA at pH7.4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | H 3 |
| Unit cell lengths | 122.105, 122.105, 42.546 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 60.000 - 2.280 |
| R-factor | 0.20886 |
| Rwork | 0.205 |
| R-free | 0.28680 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.749 |
| Data reduction software | Coot |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 60.000 |
| High resolution limit [Å] | 2.280 |
| Number of reflections | 10700 |
| <I/σ(I)> | 34.1 |
| Completeness [%] | 99.6 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 2.0M ammonium sulfate, 50mM sodium citrate pH 4.6, 5% PEG 400 |
