4X17
JC Mad-1 polyomavirus VP1 in complex with GD1b oligosaccharide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-07-12 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 149.740, 95.390, 128.880 |
Unit cell angles | 90.00, 110.40, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.750 |
R-factor | 0.16097 |
Rwork | 0.160 |
R-free | 0.18149 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3nxd |
RMSD bond length | 0.008 |
RMSD bond angle | 1.373 |
Data reduction software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0025) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.800 |
High resolution limit [Å] | 1.750 | 1.750 |
Number of reflections | 170305 | |
<I/σ(I)> | 15.5 | 2.4 |
Completeness [%] | 99.6 | 96.2 |
Redundancy | 5.7 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES pH 7.5, 0.2 M KSCN, 12% PEG 3350 |