4X15

JC Mad-1 polyomavirus VP1 in complex with GM2 oligosaccharide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-08-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	150.490, 95.680, 129.720
Unit cell angles	90.00, 110.30, 90.00

Refinement procedure

Resolution	30.000 - 2.110
R-factor	0.17387
Rwork	0.172
R-free	0.20499
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3nxg
RMSD bond length	0.008
RMSD bond angle	1.324
Data reduction software	XDS
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.160
High resolution limit [Å]	2.110	2.110
Number of reflections	99141
<I/σ(I)>	9.1	2.3
Completeness [%]	99.3	99.3
Redundancy	4.2	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M HEPES pH 7.5, 0.2 M KSCN, 12% PEG 3350