4X0L
Human haptoglobin-haemoglobin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-01-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.916 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 96.599, 96.599, 132.771 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.930 - 2.050 |
| R-factor | 0.182 |
| Rwork | 0.180 |
| R-free | 0.22364 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4f4o |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.990 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.930 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.374 | |
| Number of reflections | 43170 | |
| <I/σ(I)> | 8.7 | 2.3 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 9.6 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M NaCl, 0.1 M sodium cacodylate pH 6.5 and 2 M ammonium sulphate |
