4X09
Structure of human RNase 6 in complex with sulphate anions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 27.733, 38.860, 97.965 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.122 - 1.722 |
| R-factor | 0.1944 |
| Rwork | 0.193 |
| R-free | 0.22670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hky |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.908 |
| Data reduction software | XDS (20140110) |
| Data scaling software | Aimless (0.3.11) |
| Phasing software | PHENIX (1.9_1692) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.982 | 1.780 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.028 | 0.234 |
| Number of reflections | 11717 | |
| <I/σ(I)> | 13 | 2.4 |
| Completeness [%] | 99.2 | 99 |
| Redundancy | 1.96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 2M AMMONIUM SULPHATE, 0.1M SODIUM, CACODYLATE, 0.05M SODIUM CHLORIDE, PH 6.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293 K |
