4WZS
Crystal structure of the Mot1 N-terminal domain in complex with TBP and NC2 bound to a promoter DNA fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9794 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 150.629, 140.274, 90.778 |
| Unit cell angles | 90.00, 113.70, 90.00 |
Refinement procedure
| Resolution | 49.170 - 3.780 |
| R-factor | 0.2362 |
| Rwork | 0.235 |
| R-free | 0.25750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3oc3 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.750 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.2) |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.170 | 4.000 |
| High resolution limit [Å] | 3.780 | 3.780 |
| Rmerge | 0.100 | 0.790 |
| Number of reflections | 17169 | |
| <I/σ(I)> | 7.3 | 1.49 |
| Completeness [%] | 98.2 | 93.5 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.1 | 295 | 0.2M imidazole malate, PEG 4000 |
