4WZQ

Mutant K20E of RNA dependent RNA polymerase 3D from Foot-and-Mouth disease Virus complexed with RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.5
Spacegroup name	P 32 2 1
Unit cell lengths	95.160, 95.160, 101.329
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.160 - 2.800
R-factor	0.25052
Rwork	0.249
R-free	0.27087
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wne
RMSD bond length	0.004
RMSD bond angle	0.688
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	REFMAC (rigid body refinement using PDB id 1wne as a model)
Refinement software	REFMAC (5.8.0069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.160	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.080	0.514
Number of reflections	13460
<I/σ(I)>	11.9	2.8
Completeness [%]	99.9	100
Redundancy	3.8	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	30%PEG 4000, 0.2M Mg(CH3COO)2, 0.1M MES pH6.0